Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- M01751-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for ALIX/PDCD6IP detection. Tested with WB, IHC-P, FCM, ICC/IF in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 14D10
- Vial size
- 100μg/vial
- Storage
- At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquoted and stored frozen at -20ËšC for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization.
Yuan Q, Yang M, Zheng H, Cai Y, Luo P, Wang X, Xu P
ACS biomaterials science & engineering 2024 May 13;10(5):3355-3377
ACS biomaterials science & engineering 2024 May 13;10(5):3355-3377
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image