Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56076 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56076, RRID:AB_837611
- Product name
- Rabbit Polyclonal ASC2 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. In immuhistochemical studies, ASC-2/POP1 has been found to expressed predominantly in macrophages and granulocytes (Stehlik et al, 2003).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The PAAD/PYRIN-only protein POP1/ASC2 is a modulator of ASC-mediated nuclear-factor-kappa B and pro-caspase-1 regulation.
Stehlik C, Krajewska M, Welsh K, Krajewski S, Godzik A, Reed JC
The Biochemical journal 2003 Jul 1;373(Pt 1):101-13
The Biochemical journal 2003 Jul 1;373(Pt 1):101-13
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASC2 Antibody [NB100-56076] - Used to analyze ASC2/POP1 . HEK-293 cells were transiently transfected with plasmids encoding ASC-2, POP1 or a negative control plasmid. ASC-2 and POP1 were detected at the expected 12 kDa molecular weight.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2/POP1 expression in mouse. A) prostate tissue from a male mouse prostatitis. B) kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2 expression in mouse spleen. A and A1, spleen at low and high magnification, respectively. ASC-2 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
