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- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 11547-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11547-1-AP, RRID:AB_2245857
- Product name
- DGKA antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated DGKA antibody (Cat. #11547-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Dysfunctional natural killer cells can be reprogrammed to regain anti-tumor activity.
Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma.
Ablation of DGKα facilitates α-smooth muscle actin expression via the Smad and PKCδ signaling pathways during the acute phase of CCl(4) -induced hepatic injury.
Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.
Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.
Selective inhibitors of bromodomain BD1 and BD2 of BET proteins modulate radiation-induced profibrotic fibroblast responses.
Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade.
Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness.
Diacylglycerol kinase α inhibition cooperates with PD-1-targeted therapies to restore the T cell activation program.
Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.
DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.
Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.
Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.
Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.
Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers.
A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.
Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.
Sabag B, Puthenveetil A, Levy M, Joseph N, Doniger T, Yaron O, Karako-Lampert S, Lazar I, Awwad F, Ashkenazi S, Barda-Saad M
The EMBO journal 2024 Jul;43(13):2552-2581
The EMBO journal 2024 Jul;43(13):2552-2581
Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma.
Liu W, Wang H, Zhao Q, Tao C, Qu W, Hou Y, Huang R, Sun Z, Zhu G, Jiang X, Fang Y, Gao J, Wu X, Yang Z, Ping R, Chen J, Yang R, Chu T, Zhou J, Fan J, Tang Z, Yang D, Shi Y
Cancer communications (London, England) 2024 Feb;44(2):226-250
Cancer communications (London, England) 2024 Feb;44(2):226-250
Ablation of DGKα facilitates α-smooth muscle actin expression via the Smad and PKCδ signaling pathways during the acute phase of CCl(4) -induced hepatic injury.
Seino K, Nakano T, Tanaka T, Hozumi Y, Topham MK, Goto K, Iseki K
FEBS open bio 2024 Feb;14(2):300-308
FEBS open bio 2024 Feb;14(2):300-308
Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.
Velnati S, Centonze S, Rossino G, Purghè B, Antona A, Racca L, Mula S, Ruffo E, Malacarne V, Malerba M, Manfredi M, Graziani A, Baldanzi G
Frontiers in immunology 2023;14:1043603
Frontiers in immunology 2023;14:1043603
Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.
Zhou D, Liu T, Rao X, Jie X, Chen Y, Wu Z, Deng H, Zhang D, Wang J, Wu G
Thoracic cancer 2023 May;14(13):1179-1191
Thoracic cancer 2023 May;14(13):1179-1191
Selective inhibitors of bromodomain BD1 and BD2 of BET proteins modulate radiation-induced profibrotic fibroblast responses.
Liu CS, Rioja I, Bakr A, Veldwijk MR, Sperk E, Herskind C, Weichenhan D, Prinjha RK, Plass C, Schmezer P, Popanda O
International journal of cancer 2022 Jul 15;151(2):275-286
International journal of cancer 2022 Jul 15;151(2):275-286
Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade.
Okada N, Sugiyama K, Shichi S, Shirai Y, Goto K, Sakane F, Kitamura H, Taketomi A
Cancer immunology, immunotherapy : CII 2022 Apr;71(4):889-903
Cancer immunology, immunotherapy : CII 2022 Apr;71(4):889-903
Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness.
Manigat LC, Granade ME, Taori S, Miller CA, Vass LR, Zhong XP, Harris TE, Purow BW
Frontiers in immunology 2021;12:722469
Frontiers in immunology 2021;12:722469
Diacylglycerol kinase α inhibition cooperates with PD-1-targeted therapies to restore the T cell activation program.
Arranz-Nicolás J, Martin-Salgado M, Adán-Barrientos I, Liébana R, Del Carmen Moreno-Ortíz M, Leitner J, Steinberger P, Ávila-Flores A, Merida I
Cancer immunology, immunotherapy : CII 2021 Nov;70(11):3277-3289
Cancer immunology, immunotherapy : CII 2021 Nov;70(11):3277-3289
Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.
Arranz-Nicolás J, Martin-Salgado M, Rodríguez-Rodríguez C, Liébana R, Moreno-Ortiz MC, Leitner J, Steinberger P, Ávila-Flores A, Merida I
Journal for immunotherapy of cancer 2020 Nov;8(2)
Journal for immunotherapy of cancer 2020 Nov;8(2)
DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.
Li J, Pan C, Boese AC, Kang J, Umano AD, Magliocca KR, Yang W, Zhang Y, Lonial S, Jin L, Kang S
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Jul 15;26(14):3843-3855
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Jul 15;26(14):3843-3855
Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.
Ware TB, Franks CE, Granade ME, Zhang M, Kim KB, Park KS, Gahlmann A, Harris TE, Hsu KL
Nature chemical biology 2020 Feb;16(2):170-178
Nature chemical biology 2020 Feb;16(2):170-178
Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
Merino-Cortés SV, Gardeta SR, Roman-Garcia S, Martínez-Riaño A, Pineau J, Liebana R, Merida I, Dumenil AL, Pierobon P, Husson J, Alarcon B, Carrasco YR
Science signaling 2020 Apr 14;13(627)
Science signaling 2020 Apr 14;13(627)
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Novo D, Heath N, Mitchell L, Caligiuri G, MacFarlane A, Reijmer D, Charlton L, Knight J, Calka M, McGhee E, Dornier E, Sumpton D, Mason S, Echard A, Klinkert K, Secklehner J, Kruiswijk F, Vousden K, Macpherson IR, Blyth K, Bailey P, Yin H, Carlin LM, Morton J, Zanivan S, Norman JC
Nature communications 2018 Nov 29;9(1):5069
Nature communications 2018 Nov 29;9(1):5069
Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.
Kong Y, Zheng Y, Jia Y, Li P, Wang Y
Oncology reports 2016 Oct;36(4):1852-60
Oncology reports 2016 Oct;36(4):1852-60
Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.
Weigel C, Veldwijk MR, Oakes CC, Seibold P, Slynko A, Liesenfeld DB, Rabionet M, Hanke SA, Wenz F, Sperk E, Benner A, Rösli C, Sandhoff R, Assenov Y, Plass C, Herskind C, Chang-Claude J, Schmezer P, Popanda O
Nature communications 2016 Mar 11;7:10893
Nature communications 2016 Mar 11;7:10893
Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers.
Dominguez CL, Floyd DH, Xiao A, Mullins GR, Kefas BA, Xin W, Yacur MN, Abounader R, Lee JK, Wilson GM, Harris TE, Purow BW
Cancer discovery 2013 Jul;3(7):782-97
Cancer discovery 2013 Jul;3(7):782-97
A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.
Kefas B, Floyd DH, Comeau L, Frisbee A, Dominguez C, Dipierro CG, Guessous F, Abounader R, Purow B
Neuro-oncology 2013 Dec;15(12):1652-63
Neuro-oncology 2013 Dec;15(12):1652-63
Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Gellett AM, Kharel Y, Sunkara M, Morris AJ, Lynch KR
Biochemical and biophysical research communications 2012 Jun 15;422(4):758-63
Biochemical and biophysical research communications 2012 Jun 15;422(4):758-63
Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.
Kawaguchi M, Valencia JC, Namiki T, Suzuki T, Hearing VJ
The Journal of investigative dermatology 2012 Dec;132(12):2791-9
The Journal of investigative dermatology 2012 Dec;132(12):2791-9
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP( DGKA Antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-DGKA (IP:11547-1-AP, 3ug; Detection:11547-1-AP 1:1000) with Jurkat cells lysate 1500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The DGKA antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human DGKA. This antibody recognizes human,mouse,rat antigen. The DGKA antibody has been validated for the following applications: ELISA, IHC, IP, WB analysis.
