Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 11547-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DGKA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LEFTFKLYD TDRNGILDSS EVDKIILQMM RVAEYLDWDV SELRPILQEM MKEIDYDGSG SVSQAEWVRA GATTVPLLVL LGLEMTLKDD GQHMWRPKRF PRPVYCNLCE SSIGLGKQGL SCNLCKYTVH DQCAMKALPC EVSTYAKSRK DIGVQSHVWV RGGCESGRCD RCQKKIRIYH SLTGLHCVWC HLEIHDDCLQ AVGHECDCGL LRDHILPPSS IYPSVLASGP DRKNSKTSQK TMDDLNLSTS EALRIDPVPN THPLLVFVNP KSGGKQGQRV LWKFQYILNP RQVFNLLKDG PEIGLRLFKD VPDSRILVCG GDGTVGWILE TIDKANLPVL PPVAVLPLGT GNDLARCLRW GGGYEGQNLA KILKDLEMSK VVHMDRWS (115-501 aa encoded by BC023523)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP ( DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11547-1-AP ( DGKA antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11547-1-AP ( DGKA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DGKA (IP:11547-1-AP, 3ug; Detection:11547-1-AP 1:1000) with Jurkat cells lysate 1500ug.