Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2942 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2942, RRID:AB_1579535
- Product name
- RAGE polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RAGE.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cloning and characterization of a novel member of the MAP kinase superfamily.
A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma.
Miyata Y, Akashi M, Nishida E
Genes to cells : devoted to molecular & cellular mechanisms 1999 May;4(5):299-309
Genes to cells : devoted to molecular & cellular mechanisms 1999 May;4(5):299-309
A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma.
Gaugler B, Brouwenstijn N, Vantomme V, Szikora JP, Van der Spek CW, Patard JJ, Boon T, Schrier P, Van den Eynde BJ
Immunogenetics 1996;44(5):323-30
Immunogenetics 1996;44(5):323-30
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RAGE polyclonal antibody (Cat # PAB2942) in A-375 cell lysate. RAGE (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with RAGE polyclonal antibody (Cat # PAB2942) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)