702444

antibody from Invitrogen Antibodies

Targeting: PIP5K1C KIAA0589, LCCS3, PIP5Kgamma

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

702444

Provider

Invitrogen Antibodies

Product name

PIP5K1C Recombinant Rabbit Monoclonal Antibody (16H4L5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Pig, Mouse, Bovine Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

16H4L5

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lipid Messenger Phosphatidylinositol-4,5-Bisphosphate Is Increased by Both PPARα Activators and Inhibitors: Relevance for Intestinal Cell Differentiation.

Cizkova K, Koubova K, Tauber Z
Biology 2022 Jun 30;11(7)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, A431 cells were fixed and permeabilized for detection of endogenous Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma using Anti- PIP5K1C Recombinant Rabbit Monoclonal Antibody (Product # 702444, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localisation of PIP5K1C. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Relationships between levels of PPARalpha and PIP5K1C, PIP2, PI3K, and PTEN in HT-29 cell line. ( A ) Spearman correlation coefficients ( n = 12) for expression levels obtained with In-Cell ELISA method. PPARalpha expression under the same experimental condition was examined in our previous study []. Moderate positive correlations were found between PPARalpha and PIP5K1C and PPARalpha and PIP2, whereas PPARalpha and PTEN expression showed moderate negative correlation (statistical significance marked with asterisk, * p