Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX109939 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109939, RRID:AB_1951060
- Product name
- O-GlcNAc transferase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Tissue Engineering and Dental Implantology: Biomaterials, New Technologies, and Stem Cells.
Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V.
Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.
Dynamic behaviors of vimentin induced by interaction with GlcNAc molecules.
Sammartino G, Dohan Ehrenfest DM, Shibli JA, Galindo-Moreno P
BioMed research international 2016;2016:5713168
BioMed research international 2016;2016:5713168
Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Nagai-Okatani C, Minamino N
PloS one 2016;11(6):e0150210
PloS one 2016;11(6):e0150210
Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V.
Chen YJ, Yao CC, Huang CH, Chang HH, Young TH
BioMed research international 2015;2015:924397
BioMed research international 2015;2015:924397
Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.
Ise H, Goto M, Komura K, Akaike T
Glycobiology 2012 Jun;22(6):788-805
Glycobiology 2012 Jun;22(6):788-805
Dynamic behaviors of vimentin induced by interaction with GlcNAc molecules.
Komura K, Ise H, Akaike T
Glycobiology 2012 Dec;22(12):1741-59
Glycobiology 2012 Dec;22(12):1741-59
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects OGT protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extract7.5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: HeLa7.5% SDS PAGEGTX109939 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX109939 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
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- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract7.5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
- Validation comment
- WB
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- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 50 ?g mouse brain extract5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g PC-12 whole cell extract5% SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract5% SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody (GTX109939) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Supportive validation
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- Experimental details
- Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 £gg of O-GlcNAc transferase antibody (GTX109939).Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.