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LearnMA1-076
antibody from Invitrogen Antibodies
Targeting: OGT
FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1
Western blot
Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- MA1-076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (HGAC85)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-076 recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins. MA1-076 has been successfully used in Western blot, immunofluorescence, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects several proteins representing immunoprecipitated O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. The MA1-076 immunogen is heat killed, pepsin treated group A streptococci. DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS.
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HGAC85
- Vial size
- 100 µg
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Nutrient-Driven O-GlcNAcylation at Promoters Impacts Genome-Wide RNA Pol II Distribution.
Drosophila O-GlcNAcase Deletion Globally Perturbs Chromatin O-GlcNAcylation.
Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.
Dynamic O-GlcNAc cycling at promoters of Caenorhabditis elegans genes regulating longevity, stress, and immunity.
Glutamine enhances heat shock protein 70 expression via increased hexosamine biosynthetic pathway activity.
Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters.
Investigations into charge heterogeneity of wool intermediate filament proteins.
Glucose mediates the translocation of NeuroD1 by O-linked glycosylation.
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
Molecular alterations underlie nodal and paranodal degeneration in type 1 diabetic neuropathy and are prevented by C-peptide.
Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis.
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier.
Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.
Monoclonal antibodies to streptococcal group A carbohydrate. I. A dominant idiotypic determinant is located on Vk.
Krause MW, Love DC, Ghosh SK, Wang P, Yun S, Fukushige T, Hanover JA
Frontiers in endocrinology 2018;9:521
Frontiers in endocrinology 2018;9:521
Drosophila O-GlcNAcase Deletion Globally Perturbs Chromatin O-GlcNAcylation.
Akan I, Love DC, Harwood KR, Bond MR, Hanover JA
The Journal of biological chemistry 2016 May 6;291(19):9906-19
The Journal of biological chemistry 2016 May 6;291(19):9906-19
Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.
Tashima Y, Stanley P
The Journal of biological chemistry 2014 Apr 18;289(16):11132-42
The Journal of biological chemistry 2014 Apr 18;289(16):11132-42
Dynamic O-GlcNAc cycling at promoters of Caenorhabditis elegans genes regulating longevity, stress, and immunity.
Love DC, Ghosh S, Mondoux MA, Fukushige T, Wang P, Wilson MA, Iser WB, Wolkow CA, Krause MW, Hanover JA
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 20;107(16):7413-8
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 20;107(16):7413-8
Glutamine enhances heat shock protein 70 expression via increased hexosamine biosynthetic pathway activity.
Hamiel CR, Pinto S, Hau A, Wischmeyer PE
American journal of physiology. Cell physiology 2009 Dec;297(6):C1509-19
American journal of physiology. Cell physiology 2009 Dec;297(6):C1509-19
Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters.
Robles-Flores M, Meléndez L, García W, Mendoza-Hernández G, Lam TT, Castañeda-Patlán C, González-Aguilar H
Biochimica et biophysica acta 2008 May;1783(5):695-712
Biochimica et biophysica acta 2008 May;1783(5):695-712
Investigations into charge heterogeneity of wool intermediate filament proteins.
Paton LN, Gerrard JA, Bryson WG
Journal of proteomics 2008 Dec 2;71(5):513-29
Journal of proteomics 2008 Dec 2;71(5):513-29
Glucose mediates the translocation of NeuroD1 by O-linked glycosylation.
Andrali SS, Qian Q, Ozcan S
The Journal of biological chemistry 2007 May 25;282(21):15589-96
The Journal of biological chemistry 2007 May 25;282(21):15589-96
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
Cameron RS, Liu C, Mixon AS, Pihkala JP, Rahn RJ, Cameron PL
Cell motility and the cytoskeleton 2007 Jan;64(1):19-48
Cell motility and the cytoskeleton 2007 Jan;64(1):19-48
Molecular alterations underlie nodal and paranodal degeneration in type 1 diabetic neuropathy and are prevented by C-peptide.
Sima AA, Zhang W, Li ZG, Murakawa Y, Pierson CR
Diabetes 2004 Jun;53(6):1556-63
Diabetes 2004 Jun;53(6):1556-63
Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis.
Zhu W, Leber B, Andrews DW
The EMBO journal 2001 Nov 1;20(21):5999-6007
The EMBO journal 2001 Nov 1;20(21):5999-6007
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier.
Zhang X, Bennett V
The Journal of biological chemistry 1996 Dec 6;271(49):31391-8
The Journal of biological chemistry 1996 Dec 6;271(49):31391-8
Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.
Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW
The Journal of cell biology 1987 May;104(5):1157-64
The Journal of cell biology 1987 May;104(5):1157-64
Monoclonal antibodies to streptococcal group A carbohydrate. I. A dominant idiotypic determinant is located on Vk.
Nahm MH, Clevinger BL, Davie JM
Journal of immunology (Baltimore, Md. : 1950) 1982 Oct;129(4):1513-8
Journal of immunology (Baltimore, Md. : 1950) 1982 Oct;129(4):1513-8
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of OGT was performed using 70% confluent log phase Panc-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OGT (HGAC85) Mouse Monoclonal Antibody (Product # MA1-076) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
