LS-C775689

antibody from LSBio

Targeting: OGT FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C775689

Provider

LSBio

Product name

OGT / O-GLCNAC Antibody (clone 9H6, RPE) LS-C775689

Antibody type

Monoclonal

Description

PEG purified

Host

Mouse

Isotype

IgM

Antibody clone number

9H6

Storage

Store at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Citrulline Antibody (C) Composite.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Citrulline Antibody (C) Composite.