Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-32791 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32791, RRID:AB_2157069
- Product name
- Rabbit Polyclonal O-GlcNAc Transferase p110 subunit Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.
Ise H, Goto M, Komura K, Akaike T
Glycobiology 2012 Jun;22(6):788-805
Glycobiology 2012 Jun;22(6):788-805
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug PC-12 whole cell extract 5 % SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 ug of O-GlcNAc transferase antibody. Western blot analysis was performed using O-GlcNAc transferase antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody diluted at 1:500.