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LearnPAB28527
antibody from Abnova Corporation
Targeting: CHEK2
bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB28527 - Provider product page
- Provider
- Abnova Corporation
- Product name
- CHEK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant CHEK2.
- Antigen sequence
TSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPED
QEPEDQEPEEPTPAPWARLWALQDGFANLECVNDN
YWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIF
REVGPKNSYIAYIEDHSGNG- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CHEK2 polyclonal antibody (Cat # PAB28527) at 1:100-1:250 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human colon with CHEK2 polyclonal antibody (Cat # PAB28527) shows strong nuclear positivity in glandular cells at 1:10-1:20 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
