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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
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- Product number
- AF1626 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Chk2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Chk2 when phosphorylated at T68.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references WRN helicase is a synthetic lethal target in microsatellite unstable cancers.
Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, Gonzalez A, McPartlan JS, Li T, Zhang Y, Bin Liu J, Lazaro JB, Gu P, Piett CG, Apffel A, Ali SO, Deasy R, Keskula P, Ng RWS, Roberts EA, Reznichenko E, Leung L, Alimova M, Schenone M, Islam M, Maruvka YE, Liu Y, Roper J, Raghavan S, Giannakis M, Tseng YY, Nagel ZD, D'Andrea A, Root DE, Boehm JS, Getz G, Chang S, Golub TR, Tsherniak A, Vazquez F, Bass AJ
Nature 2019 Apr;568(7753):551-556
Nature 2019 Apr;568(7753):551-556
Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.
Shi Y, Felley-Bosco E, Marti TM, Orlowski K, Pruschy M, Stahel RA
BMC cancer 2012 Dec 4;12:571
BMC cancer 2012 Dec 4;12:571
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Boichuk S, Hu L, Hein J, Gjoerup OV
Journal of virology 2010 Aug;84(16):8007-20
Journal of virology 2010 Aug;84(16):8007-20
RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.
Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M
The Journal of cell biology 2009 May 18;185(4):587-600
The Journal of cell biology 2009 May 18;185(4):587-600
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
Journal of virology 2009 Jan;83(1):117-27
Journal of virology 2009 Jan;83(1):117-27
Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
Leonard JM, Ye H, Wetmore C, Karnitz LM
The Journal of cell biology 2008 Nov 3;183(3):385-91
The Journal of cell biology 2008 Nov 3;183(3):385-91
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Sakasai R, Tibbetts R
The Journal of biological chemistry 2008 May 16;283(20):13549-55
The Journal of biological chemistry 2008 May 16;283(20):13549-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
