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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX70100 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70100, RRID:AB_11177978
- Product name
- Chk2 antibody [4B8]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHEK2 antibody detects CHEK2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHEK2 antibody (GTX70100) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] (GTX70100) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] (GTX70100) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Chk2 protein from 293T whole cell extracts using 5 £gg of Chk2 antibody (GTX70100).Western blot analysis was performed using Chk2 antibody (GTX70100).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
