GTX70295

antibody from GeneTex

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX70295

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70295, RRID:AB_369315

Product name

Chk2 antibody [8F12]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

ATM directs DNA damage responses and proteostasis via genetically separable pathways.

Lee JH, Mand MR, Kao CH, Zhou Y, Ryu SW, Richards AL, Coon JJ, Paull TT
Science signaling 2018 Jan 9;11(512)

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Homeodomain Proteins Directly Regulate ATM Kinase Activity.

Johnson TE, Lee JH, Myler LR, Zhou Y, Mosley TJ, Yang SH, Bio-Bricks for Molecular Machines FRI Stream, Uprety N, Kim J, Paull TT
Cell reports 2018 Aug 7;24(6):1471-1483

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Direct activation of ATM by resveratrol under oxidizing conditions.

Lee JH, Guo Z, Myler LR, Zheng S, Paull TT
PloS one 2014;9(6):e97969

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A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.

Chen HM, Chang FR, Hsieh YC, Cheng YJ, Hsieh KC, Tsai LM, Lin AS, Wu YC, Yuan SS
Free radical biology & medicine 2011 May 1;50(9):1151-62

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (GTX70295) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using (GTX70295) antibody at 1:500 dilution.