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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- GTX84685 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84685, RRID:AB_10726750
- Product name
- Chk2 antibody [2F4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-CHEK2 mouse monoclonal antibody (GTX84685) immunofluorescent staining of COS7 cells transiently transfected with CHEK2
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (GTX84685).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either CHEK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (GTX84685), and then analyzed by flow cytometry.
