GTX84688

antibody from GeneTex

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84688

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84688, RRID:AB_10727427

Product name

Chk2 antibody [5C7]

Antibody type

Monoclonal

Reactivity

Human, Canine

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CHEK2mouse monoclonal antibody. (GTX84688, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon tissue using anti-CHEK2mouse monoclonal antibody. (GTX84688, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue using anti-CHEK2mouse monoclonal antibody. (GTX84688, Dilution 1:50)

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells transfected with either CHEK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (GTX84688), and then analyzed by flow cytometry.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-CHEK2 antibody (GTX84688), (Red), compared to a nonspecific negative control antibody, (Blue).