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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX84689 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84689, RRID:AB_10727793
- Product name
- Chk2 antibody [10G7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (GTX84689).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-CHEK2mouse monoclonal antibody. (GTX84689, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-CHEK2mouse monoclonal antibody. (GTX84689, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either CHEK2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (GTX84689), and then analyzed by flow cytometry.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-CHEK2 antibody (GTX84689), (Red), compared to a nonspecific negative control antibody, (Blue).
