H00011200-M01
antibody from Novus Biologicals
Targeting: CHEK2
bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00011200-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011200-M01, RRID:AB_537739
- Product name
- Mouse Monoclonal Chk2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CHEK2 - CHK2 checkpoint homolog (S. pombe)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human Kinase/Phosphatase-Wide RNAi Screening Identified Checkpoint Kinase 2 as a Cellular Factor Facilitating Japanese Encephalitis Virus Infection.
Application of immunohistochemical staining to detect antigen destruction as a measure of tissue damage.
Part II. Initial molecular and cellular characterization of high nitric oxide-adapted human tongue squamous cell carcinoma cell lines.
Chan YL, Liao CL, Lin YL
Frontiers in cellular and infection microbiology 2018;8:142
Frontiers in cellular and infection microbiology 2018;8:142
Application of immunohistochemical staining to detect antigen destruction as a measure of tissue damage.
Onul A, Colvard MD, Paradise WA, Elseth KM, Vesper BJ, Gouvas E, Deliu Z, Garcia KD, Pestle WJ, Radosevich JA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2012 Sep;60(9):683-93
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2012 Sep;60(9):683-93
Part II. Initial molecular and cellular characterization of high nitric oxide-adapted human tongue squamous cell carcinoma cell lines.
Tarjan G, Haines GK 3rd, Vesper BJ, Xue J, Altman MB, Yarmolyuk YR, Khurram H, Elseth KM, Roeske JC, Aydogan B, Radosevich JA
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2011 Feb;32(1):87-98
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2011 Feb;32(1):87-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.