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LearnPAB10034
antibody from Abnova Corporation
Targeting: CHEK2
bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB10034 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10034, RRID:AB_1707209
- Product name
- CHEK2 (phospho T68) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage.
A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase.
Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
Brown AL, Lee CH, Schwarz JK, Mitiku N, Piwnica-Worms H, Chung JH
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3745-50
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3745-50
A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase.
Blasina A, de Weyer IV, Laus MC, Luyten WH, Parker AE, McGowan CH
Current biology : CB 1999 Jan 14;9(1):1-10
Current biology : CB 1999 Jan 14;9(1):1-10
Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
Matsuoka S, Huang M, Elledge SJ
Science (New York, N.Y.) 1998 Dec 4;282(5395):1893-7
Science (New York, N.Y.) 1998 Dec 4;282(5395):1893-7
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using CHEK2 (phospho T68) polyclonal antibody (Cat # PAB10034) shows detection of apredominant band at ~60 kDa corresponding to phosphorylated CHEK2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.CHEK2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production.Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
