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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500405 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500405, RRID:AB_2080659
- Product name
- CHEK2 (CHK2) mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
- Antibody type
- Monoclonal
- Description
- CHEK2 (CHK2) mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CHEK2
- Isotype
- IgG
- Antibody clone number
- OTI2F4
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CHEK2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-CHEK2 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CHEK2 mouse monoclonal antibody (TA500405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500405), and then analyzed by flow cytometry.
- Validation comment
- FC
