ABIN362141

antibody from antibodies-online

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Recommended by provider)

Antibody Data

Product number

ABIN362141

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN362141, RRID:AB_10953756

Product name

anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer516 (Q-P-S (p) -T-S) derived from Human Chk2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human

Host

Rabbit

Epitope

pSer516

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis.

Wu X, Chen J
The Journal of biological chemistry 2003 Sep 19;278(38):36163-8

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Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation.

Schwarz JK, Lovly CM, Piwnica-Worms H
Molecular cancer research : MCR 2003 Jun;1(8):598-609

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MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.

Lou Z, Minter-Dykhouse K, Wu X, Chen J
Nature 2003 Feb 27;421(6926):957-61