AMM03384G

antibody from Leading Biology
Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Provider product page for AMM03384G
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
AMM03384G - Provider product page
Provider
Leading Biology
Product name
CHEK2 Antibody (monoclonal) (M01)
Antibody type
Monoclonal
Description
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
4B7
Storage
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
CHEK2 protein structure - AMM03384G shown in red.