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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- RQ8192 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Checkpoint kinase 2 Antibody / Chk2 / CHEK2
- Antibody type
- Polyclonal
- Description
- This highly specific Checkpoint kinase 2 antibody is suitable for use in Western blot/Immunofluorescence/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Checkpoint kinase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) MCF7 and 3) 293T cell lysate with Checkpoint kinase 2 antibody. Predicted molecular weight ~61 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with Checkpoint kinase 2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
