GTX128073

antibody from GeneTex

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX128073

Provider

GeneTex

Product name

Chk2 (phospho Thr68) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein by Western blot analysis.A. 30 μg U2OS whole cell lysate/extract (untreat)B. 30 μg U2OS whole cell lysate/extract (UVC-treated 100 J/m2 and recover for 2hr)C. 30 μg U2OS whole cell lysate/extract (UVC-treated 100 J/m2 and recover for 6hr)7.5 % SDS-PAGEChk2 (phospho Thr68) antibody (GTX128073) dilution: 1:250

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein by western blot analysis. Un-treated (-) and treated (+, 100 £gM etoposide treatment for 2 and 6 hr) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (GTX128073) diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Chk2 (phospho Thr68) antibody (GTX128073) dilution: 1:500.