AF3697

antibody from Novus Biologicals

Targeting: PTPRT KIAA0283, RPTPrho

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3697

Provider

Novus Biologicals

Product name

Goat Polyclonal PTP rho/PTPRT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PTP rho/PTPRT in Western blots. In Western blots, no cross-reactivity with recombinant human LAR, PTPRM, K, S, G or DEP1/CD148 is observed.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PTP rho/PTPRT by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTP rho/PTPRT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTP rho/PTPRT at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.