Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B8327 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ GDF15 Antibody (C-Terminus, clone 6D12.H10.E4) LS-B8327
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 6D12.H10.E4
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Anti-GDF15 antibody (LS-B8327, 1 µg/ml; 40 ng per lane). Lane 1: Protein Standard Opal Pre-stained. Lane 2: MBP-GDF15 fusion protein 1. Lane 3: MBP—GDF15 fusion protein 2. Lane 4: MBP. Antibody produced band at ~63 kDa (MBP+GDF15 fusion, predicted MW ~60kDa).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Anti-GDF15 antibody (LS-B8327, 1 µg/ml). Lane 1: Protein Standard Opal Pre-stained. Lane 2: HeLa lysate [10 µl] + recomb. GDF15 [0.05 µg]. Lane 3: HeLa lysate [10 µl] + recomb. GDF15 [0.02 µg]. Lane 4: HeLa lysate [10 µl] + recomb. GDF15 [0.01 µg]. Lane 5: HeLa lysate [10 µl]. Lane 6: HeLa lysate [10 µl] + MBP [0.05 µg]. Antibody produced band at ~17 kDa (predicted MW: 17 kDA).