Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C154010 - Provider product page
- Provider
- LSBio
- Product name
- GDF15 Antibody (clone 7C12.B3.F2) LS-C154010
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7C12.B3.F2
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NAG-1 (H variant specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.