TA319181

antibody from OriGene

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319181

Provider

OriGene

Product name

Rabbit polyclonal Nag-1 antibody Biotin Conjugated

Antibody type

Polyclonal

Description

Rabbit polyclonal Nag-1 antibody Biotin Conjugated

Host

Rabbit

Conjugate

Unconjugated

Epitope

GDF15

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 6 . Negative control of human colon tissue pH6 is shown in background. Tissue: Human Colon

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 9 . Negative control of human colon tissue pH9 is shown in background. Tissue: Human Colon

Validation comment

IHC