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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319546 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal NAG-1 H Variant antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal NAG-1 H Variant antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GDF15
- Antibody clone number
- 7C12.B3.F2
- Vial size
- 100 µg
- Concentration
- 1.33 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. For detection, peroxidase conjugated Gt-a-Mouse IgG was used at 1:40000.
- Validation comment
- WB
