Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319470 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-NAG-1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-NAG-1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Gdf15
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.13 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody.
- Validation comment
- WB