A01583-3

antibody from Boster Biological Technology

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01583-3

Provider

Boster Biological Technology

Product name

Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for GDF15 detection. Tested positive for ELISA,Immunohistochemistry,Western Blot, in human, mouse

Reactivity

Human, Mouse

Host

Rabbit

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

References

Submitted references

Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis.

Wang Y, Li D, Xu N, Tao W, Zhu R, Sun R, Fan W, Zhang P, Dong T, Yu L
Arthritis research & therapy 2011;13(6):R193

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of GDF15 using anti-GDF15 antibody (A01583-3).GDF15 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GDF15 Antibody (A01583-3) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image