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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C745241 - Provider product page
- Provider
- LSBio
- Product name
- GDF15 Antibody LS-C745241
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-Human NAG-1 antibody shows detection of a 42 kDa band corresponding to purified recombinant fusion protein MBP-human NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1:1000 in 1% BSA-TBST overnight at 4°C. DyLight649 Goat Anti-Rabbit IgG secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.
