Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- LS-C745322 - Provider product page
- Provider
- LSBio
- Product name
- GDF15 Antibody LS-C745322
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti NAG1 antibody. Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti NAG1 antibody. Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti NAG1 antibody. Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti NAG1 antibody. Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.