Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C744826 - Provider product page
- Provider
- LSBio
- Product name
- GDF15 Antibody (clone 23B3.D2.H5) LS-C744826
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23B3.D2.H5
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag, lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag, lane 3 - yeast cell lysate expressing NAG-1 H variant and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins correspond to 32 kDa and 16 kDa bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Detection occurred using a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.