PA5-120025

antibody from Invitrogen Antibodies

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120025

Provider

Invitrogen Antibodies

Product name

GDF15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: PC-3, HT-1080. The target is usually found in the following locations: Secreted. Immunogen sequence: EDSRFRELRK RYEDLLTRLR ANQSWEDSNT DLVPAPAVRI LTPEVRLGSG GHLHLRISRA ALPEGLPEAS RLHRALFRLS PTASRSWDVT RPLRRQLSLA RPQAPALHLR LSPPPSQSDQ LLAESSSARP QLELHLRPQA ARGRRRARAR NGDHCPLGPG RCCRLHTVRA SLEDLGWADW VLSPREVQVT MCIGACPSQF RAANMHAQIK TSLHRLKPDT VPAPCCVPAS YNPMVLIQKT DTGVSLQTYD DLLAKDCHCI

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.63 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GDF15 in BALB-3T3 cells. Samples were incubated with GDF15 Polyclonal antibody (Product # PA5-120025) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GDF15 in 200 μg extracts of HT-1080 cells. Samples were precipitated with 3 μg GDF15 Polyclonal antibody (Product # PA5-120025). Western blot was performed from the immunoprecipitate using GDF15 Polyclonal antibody (Product # PA5-120025) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GDF15 in paraffin-embedded human placenta. Samples were incubated with GDF15 Polyclonal antibody (Product # PA5-120025) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.