PA5-120025
antibody from Invitrogen Antibodies
Targeting: GDF15
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-120025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDF15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: PC-3, HT-1080. The target is usually found in the following locations: Secreted. Immunogen sequence: EDSRFRELRK RYEDLLTRLR ANQSWEDSNT DLVPAPAVRI LTPEVRLGSG GHLHLRISRA ALPEGLPEAS RLHRALFRLS PTASRSWDVT RPLRRQLSLA RPQAPALHLR LSPPPSQSDQ LLAESSSARP QLELHLRPQA ARGRRRARAR NGDHCPLGPG RCCRLHTVRA SLEDLGWADW VLSPREVQVT MCIGACPSQF RAANMHAQIK TSLHRLKPDT VPAPCCVPAS YNPMVLIQKT DTGVSLQTYD DLLAKDCHCI
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GDF15 in BALB-3T3 cells using a GDF15 Polyclonal antibody (Product # PA5-120025). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GDF15 in BALB-3T3 cells. Samples were incubated with GDF15 Polyclonal antibody (Product # PA5-120025) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GDF15 in 200 μg extracts of HT-1080 cells. Samples were precipitated with 3 μg GDF15 Polyclonal antibody (Product # PA5-120025). Western blot was performed from the immunoprecipitate using GDF15 Polyclonal antibody (Product # PA5-120025) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GDF15 in paraffin-embedded human placenta. Samples were incubated with GDF15 Polyclonal antibody (Product # PA5-120025) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.