PAB16901

antibody from Abnova Corporation

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB16901

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB16901, RRID:AB_10677883

Product name

Gdf15 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against full length recombinant Gdf15.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Changes in gene expression contribute to cancer prevention by COX inhibitors.

Baek SJ, Eling TE
Progress in lipid research 2006 Jan;45(1):1-16

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H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.

Lindmark F, Zheng SL, Wiklund F, Bensen J, Bälter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Grönberg H, Xu J
Journal of the National Cancer Institute 2004 Aug 18;96(16):1248-54

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using Gdf15 polyclonal antibody (Cat # PAB16901). The blot shows detection of recombinant MBP-Gdf15 fusion protein (60 KDa) purified from E.coli (Lane 1) ; yeast cell lysate expressing SUMO-mouse Gdf15 (42 KDa) (Lane 2), and R&D human Gdf15 monomer purified from CHO-K1 cells (14 KDa) (Lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.