NBP1-42719

antibody from Novus Biologicals

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

NBP1-42719

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-42719, RRID:AB_10005906

Product name

Mouse Monoclonal GDF-15 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Partial reactivity is expected against rat based on an 86% identity with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.13 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GDF-15 Antibody (23B3D2.H5) [NBP1-42719] - Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins without SUMO correspond to monomer (15 kDa) and dimer (30 kDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.