PA5-29782

antibody from Invitrogen Antibodies

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Antibody data

Submit

Validation data

GDF15 protein structure - PA5-29782 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GDF-15 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GDF-15 polyclonal antibody (Product # PA5-29782) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using GDF15 Polyclonal Antibody (Product # PA5-29782). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GDF15 Polyclonal Antibody (Product # PA5-29782) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-GDF15 Polyclonal Antibody (Product # PA5-29782) and a ~34kDa band corresponding to GDF15 was observed across the cell lines tested except Jurkat which is reported to be negative. The expression of protein also increased in HCT 116 upon thapsigargin treatment. Whole cell extracts (30ug lysate) of LNCaP (Lane 1), LNCaP treated with PTI (1X for 4 hr) (Lane2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X for 4hr) (Lane 4), HCT 116 treated with thapsigargin (16uM for 16hr) and PTI (1X for 4 hr) (Lane 5), Jurkat (Lane 6) and Jurkat treated with PTI (1X for 4hr) (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).