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LearnAP09407PU-S
antibody from Acris Antibodies GmbH
Targeting: GDF15
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09407PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09407PU-S, RRID:AB_2035523
- Product name
- anti GDF15 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to the C-terminal sequence of NAG-1 protein. A residue of cysteine was added to facilitate coupling
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
