AP09408PU-N

antibody from Acris Antibodies GmbH

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09408PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09408PU-N, RRID:AB_2035519

Product name

anti GDF15 (H variant)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to an H-variant sequence located at the amino terminus of mature Human NAG-1 protein. A residue of cysteine was added to facilitate coupling

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

In this Sandwich ELISA, NAG-1 was captured from Human serum using the following antibodies: anti-NAG-1/GDF15 (C-terminal specific), anti-NAG-1/GDF15 (N-terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with Capture antibody at 1 µg/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37°C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.