Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- AMAb90687 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90687, RRID:AB_2665632
- Product name
- Anti-GDF15
- Antibody type
- Monoclonal
- Description
- Monoclonal Antibody against Human GDF15, Clone ID: CL0328, Gene description: growth differentiation factor 15, Alternative Gene Names: MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB, Validated applications: ICC, IHC, WB, Uniprot ID: Q99988, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CL0328
- Vial size
- 100 µl
- Concentration
- 1.89 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references ZBTB18 regulates cytokine expression and affects microglia/macrophage recruitment and commitment in glioblastoma
DHODH inhibition modulates glucose metabolism and circulating GDF15, and improves metabolic balance
The interplay of growth differentiation factor 15 (GDF15) expression and M2 macrophages during prostate carcinogenesis
Ferrarese R, Joseph K, Andrieux G, Haase I, Zanon F, Kling E, Izzo A, Corrales E, Schwabenland M, Prinz M, Ravi V, Boerries M, Heiland D, Carro M
Communications Biology 2024;7(1)
Communications Biology 2024;7(1)
DHODH inhibition modulates glucose metabolism and circulating GDF15, and improves metabolic balance
Zhang J, Terán G, Popa M, Madapura H, Ladds M, Lianoudaki D, Grünler J, Arsenian-Henriksson M, McCormack E, Rottenberg M, Catrina S, Laín S, Darekar S
iScience 2021;24(5):102494
iScience 2021;24(5):102494
The interplay of growth differentiation factor 15 (GDF15) expression and M2 macrophages during prostate carcinogenesis
Sadasivan S, Chen Y, Gupta N, Han X, Bobbitt K, Chitale D, Williamson S, Rundle A, Tang D, Rybicki B
Carcinogenesis 2020;41(8):1074-1082
Carcinogenesis 2020;41(8):1074-1082
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of A549 cells using the Anti-GDF15 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb90687 antibody. Corresponding GDF15 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol