Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- HPA011191 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA011191, RRID:AB_1078962
- Product name
- Anti-GDF15
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLL
TRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGG
HLHLRISRAALPEGLPEASRLHRALFRLSPTASRS
WDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQL- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells.
Association of serum level of growth differentiation factor 15 with liver cirrhosis and hepatocellular carcinoma.
The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis.
GDF15 derived from both tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression via Akt and Erk pathways
GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells.
Growth differentiation factor 15: a prognostic marker for recurrence in colorectal cancer.
GDF-15 Contributes to Proliferation and Immune Escape of Malignant Gliomas
Primary Ovarian Mucinous Carcinoma of Intestinal Type: Significance of Pattern of Invasion and Immunohistochemical Expression Profile in a Series of 31 Cases
Lee J, Fricke F, Warnken U, Schnölzer M, Kopitz J, Gebert J
PloS one 2015;10(6):e0131506
PloS one 2015;10(6):e0131506
Association of serum level of growth differentiation factor 15 with liver cirrhosis and hepatocellular carcinoma.
Liu X, Chi X, Gong Q, Gao L, Niu Y, Chi X, Cheng M, Si Y, Wang M, Zhong J, Niu J, Yang W
PloS one 2015;10(5):e0127518
PloS one 2015;10(5):e0127518
The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis.
Uchiyama T, Kawabata H, Miura Y, Yoshioka S, Iwasa M, Yao H, Sakamoto S, Fujimoto M, Haga H, Kadowaki N, Maekawa T, Takaori-Kondo A
Cancer medicine 2015 Oct;4(10):1558-72
Cancer medicine 2015 Oct;4(10):1558-72
GDF15 derived from both tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression via Akt and Erk pathways
Urakawa N, Utsunomiya S, Nishio M, Shigeoka M, Takase N, Arai N, Kakeji Y, Koma Y, Yokozaki H
Laboratory Investigation 2015 March;95(5):491-503
Laboratory Investigation 2015 March;95(5):491-503
GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells.
Nickel N, Jonigk D, Kempf T, Bockmeyer CL, Maegel L, Rische J, Laenger F, Lehmann U, Sauer C, Greer M, Welte T, Hoeper MM, Golpon HA
Respiratory research 2011 May 6;12(1):62
Respiratory research 2011 May 6;12(1):62
Growth differentiation factor 15: a prognostic marker for recurrence in colorectal cancer.
Wallin U, Glimelius B, Jirström K, Darmanis S, Nong RY, Pontén F, Johansson C, Påhlman L, Birgisson H
British journal of cancer 2011 May 10;104(10):1619-27
British journal of cancer 2011 May 10;104(10):1619-27
GDF-15 Contributes to Proliferation and Immune Escape of Malignant Gliomas
Roth P, Junker M, Tritschler I, Mittelbronn M, Dombrowski Y, Breit S, Tabatabai G, Wick W, Weller M, Wischhusen J
Clinical Cancer Research 2010 July;16(15):3851-3859
Clinical Cancer Research 2010 July;16(15):3851-3859
Primary Ovarian Mucinous Carcinoma of Intestinal Type: Significance of Pattern of Invasion and Immunohistochemical Expression Profile in a Series of 31 Cases
Tabrizi A, Kalloger S, Köbel M, Cipollone J, Roskelley C, Mehl E, Gilks C
International Journal of Gynecological Pathology 2010 ;29(2):99-107
International Journal of Gynecological Pathology 2010 ;29(2):99-107
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
- Sample type
- HUMAN
Enhanced validation
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and cervix, uterine tissues using HPA011191 antibody. Corresponding GDF15 RNA-seq data are presented for the same tissues.
- Sample type
- HUMAN
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows high expression.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows low expression as expected.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in a small subset of glandular cells.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows very strong cytoplasmic positivity in trophoblastic cells.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cervix shows no positivity in squamous epithelial cells as expected.
- Sample type
- HUMAN