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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-60121 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ISCA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DKLSSEFVFN NPNIKGTCGC GESFNI
- Concentration
- 0.2 mg/mL
Submitted references Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.
Expanding the phenotype of mitochondrial disease: Novel pathogenic variant in ISCA1 leading to instability of the iron-sulfur cluster in the protein.
Ling Y, Ma J, Qi X, Zhang X, Kong Q, Guan F, Dong W, Chen W, Gao S, Gao X, Pan S, Ma Y, Lu D, Zhang L
Animal models and experimental medicine 2021 Dec;4(4):381-390
Animal models and experimental medicine 2021 Dec;4(4):381-390
Expanding the phenotype of mitochondrial disease: Novel pathogenic variant in ISCA1 leading to instability of the iron-sulfur cluster in the protein.
Lebigot E, Hully M, Amazit L, Gaignard P, Michel T, Rio M, Lombès M, Thérond P, Boutron A, Golinelli-Cohen MP
Mitochondrion 2020 May;52:75-82
Mitochondrion 2020 May;52:75-82
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ISCA1 in control (vector only transfected HEK293T lysate) and ISCA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ISCA1 Polyclonal Antibody (Product # PA5-60121).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ISCA1 in human liver tissue shows strong cytoplasmic positivity in hepatocytes. Samples were probed using an ISCA1 Polyclonal Antibody (Product # PA5-60121).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 The expression of ISCA1 under cardiomyopathy and establishment of myocardium specific Isca1 knockout heterozygote (HET) rat. (A) Phenotypic characteristics of clinical patients with Isca1 mutation. (B) ISCA1 expression in myocardium from ischemic and non-ischemic cardiomyopathy patients compared with normal donors ( p < .01, data from GSE1869 , n = 6 in normal group; n = 10 in ischemic group; n = 21 in non-ischemic group). (C) The HET ( Isca1 flox/+ /alpha-MHC-Cre ) rat was obtained by crossing Isca1 -floxed rats with alpha-MHC-Cre rats. Genotyping assays were performed using PCR (D) and Isca1 expression in the myocardium of HET rats was detected using western blot (E-F, n = 3 rats per group, * p < .05, vs. WT)
