66183-1-Ig

antibody from Proteintech Group

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

66183-1-Ig

Provider

Proteintech Group

Product name

HSP70 antibody

Antibody type

Monoclonal

Description

HSP70 antibody (Cat. #66183-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, yeast, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat, Yeast

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

4E7E5

Vial size

20ul, 150ul

References

Submitted references

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Echinacoside regulates the IRE1/XBP1 signaling pathway through HSP72 to reduce endoplasmic reticulum stress and improve diabetic kidney disease.

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Dental Pulp Stem Cell-Derived Intracellular Vesicles Promote Cartilage Regeneration and Alleviate Pain in Temporomandibular Joint Osteoarthritis.

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Extracellular vesicles derived from irradiated oral squamous cell carcinoma cells inhibits M2 macrophage polarization by the miR-198-5p/MYD88 axis.

Du X, Duan M, Zhang Y, Li R
International journal of radiation biology 2025;101(11):1117-1128

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Ginsenoside Rh2 ameliorates pulmonary fibrosis via HSP70-dependent autophagic flux regulation and senescence arrest.

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Journal of ethnopharmacology 2025 Sep 25;353(Pt B):120448

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PDLIM4 drives gastric cancer malignant progression and cisplatin resistance by inhibiting HSP70 ubiquitination and degradation via competitive interaction with STUB1.

Zhu C, Chen M, Fan L, Wang Y, Liu M, Kang G, Yin F, Tang H, He Y, Zhang S, Zeng L, Liu W, Yu K, Le A
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Elesclomol-Induced Copper Influx Attenuates Lung Adenocarcinoma Progression With Involvement of the ER Stress/PCK2 Axis.

Zhao J, Chen Y, Lu D, Zeng Y, Li J, Zhu J, Lei Z, Huang JA, Liu Z
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Heat shock protein A1 inhibits the replication of foot-and-mouth disease virus by degrading viral RNA polymerase 3D through chaperone-mediated autophagy.

Ren M, Zhou H, Wu J-e, Wang J-n, Wang X, Abdullah SW, Guo H, Sun S
Journal of virology 2025 May 20;99(5):e0016825

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Microneedle-mediated hypoxic extracellular vesicle-encapsulated selenium nanoparticles delivery to treat androgenetic alopecia.

Jing S, Liu Y, Wang B, Zhou H, Zhang H, Siwakoti P, Qu X, Ye P, He Y, Kumeria T, Ye Q
Journal of controlled release : official journal of the Controlled Release Society 2025 May 10;381:113597

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Extracellular HSP70 facilitated β-glucan induced trained immunity in macrophages to suppress sepsis via TLR2-NF-κB axis.

Qi R, Cheng X, Chen S, Fan J
Cytokine 2025 Mar;187:156861

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Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia.

Liu Y, Zhou H, Pang P, Liu Y, Situ J, Wang J, He Y, Ye Q
Stem cell research & therapy 2025 Mar 31;16(1):161

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Mannose-modified exosomes loaded with MiR-23b-3p target alveolar macrophages to alleviate acute lung injury in Sepsis.

Lin J, Yang L, Liu T, Zhao H, Liu Y, Shu F, Huang H, Liu W, Zhang W, Jiang L, Xiao S, Zheng Y, Xia Z
Journal of controlled release : official journal of the Controlled Release Society 2025 Mar 10;379:832-847

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A positive feedback loop of OTUD1 and c-Jun driven by leptin expedites stemness maintenance in ovarian cancer.

Wang J, Yang F, Chen Y, Xing Y, Huang J, Cao J, Xiong J, Liu Y, Zhao Q, Luo M, Xiong J, Fan G, Lyu Q, Li F, Zhang W
Oncogene 2025 Jun;44(22):1731-1745

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Localized brain stimulation with mild magnetic hyperthermia promotes microglia activity towards reactive and autophagic phenotypes in vivo.

Jeon BT, Naveed M, Puleo M, Kim WY, Kim MH
Scientific reports 2025 Jul 8;15(1):24425

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Single-cell transcriptomic analysis of the senescent microenvironment in bone metastasis.

Wang S, Ao L, Lin H, Wei H, Wu Z, Lu S, Liang F, Shen R, Zhang H, Miao T, Shen X, Lin J, Zhong G
Cell proliferation 2025 Jan;58(1):e13743

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Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity.

Peng B, Li Z, Cheng Y, Jiang H, Ye Q, Han G
Stem cell research & therapy 2025 Jan 23;16(1):22

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A near-infrared triggered multi-functional indocyanine green nanocomposite with NO gas release function inducing improved photothermal therapy.

Guo L, Zhou Y, Ding J, Xiong J, Zhu L, Amuti S, Zhang C, Du Z, Zhang X, Dong B, Alifu N
Journal of colloid and interface science 2025 Feb;679(Pt B):307-323

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Blocking the SIRPα-CD47 axis promotes macrophage phagocytosis of exosomes derived from visceral adipose tissue and improves inflammation and metabolism in mice.

Lin YK, Pan YF, Jiang TY, Chen YB, Shang TY, Xu MY, Feng HB, Ma YH, Tan YX, Wang HY, Dong LW
Journal of biomedical science 2025 Feb 28;32(1):31

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SRF/SLC31A1 signaling promotes cuproptosis induced by celastrol in NSCLC.

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Arginyltransferase1 drives a mitochondria-dependent program to induce cell death.

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Control Over Kinetic Self-Assembly of Phthalocyanine into Photothermal Nano-Vaccine Enabling Tunable Superlarge Absorption Redshift.

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Deficiency of HSF4 Increases the Secretion of Small Extracellular Vesicles via Upregulation of Chaperone-Mediated Autophagy.

Liu J, Liu X, Xie X, Si W, Zhang Y, Xue M, Peng X, Jiang M, Du S, Shao J, Mao Y, Zhang F, Hu Y
Journal of cellular biochemistry 2025 Apr;126(4):e70031

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Gastric cancer cell-derived exosomal miRNA-128-3p promotes angiogenesis by targeting SASH1.

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Frontiers in oncology 2024;14:1440996

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GRP75 triggers white adipose tissue browning to promote cancer-associated cachexia.

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Qingfei Zhisou oral liquid alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression.

Jiaming G, Yehao Z, Yuanyuan C, Long J, Jianfeng Z, Hao G, Jianhua FU
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2024 Oct;44(5):954-962

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Yan F, Chen B, Ma Z, Chen Q, Jin Z, Wang Y, Qu F, Meng Q
Scientific reports 2024 Nov 27;14(1):29466

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Arginyltransferase1 drives a mitochondria-dependent program to induce cell death.

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bioRxiv : the preprint server for biology 2024 Nov 23;

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Alpha-Emitter Radium-223 Induces STING-Dependent Pyroptosis to Trigger Robust Antitumor Immunity.

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Disrupting the protein-protein interaction network of Hsp72 inhibits adipogenic differentiation and lipid synthesis in adipocytes.

Hu YT, Lin YW, Guo SY, Jiang Z, Xu SM, Su Z, Zhang JM, Rao Y, Chen SB, Huang ZS
Cellular signalling 2024 Dec;124:111431

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Development of Novel Silicon-Based Hydrophobic Tags (SiHyT) for Targeted Proteins Degradation.

Ma L, Zhang K, Huang Z, Guo Y, Liu N, Chen J, Wang X, Liu Y, Li M, Li J, Yang C, Liu S, Yang G
Journal of medicinal chemistry 2024 Dec 12;67(23):21344-21363

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A NRF2 Regulated and the Immunosuppressive Microenvironment Reversed Nanoplatform for Cholangiocarcinoma Photodynamic-Gas Therapy.

Wang W, Gao Y, Xu J, Zou T, Yang B, Hu S, Cheng X, Xia Y, Zheng Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Apr;11(14):e2307143

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In vitro and in vivo study on the treatment of non-small cell lung cancer with radionuclide labeled PD-L1 nanobody.

Zhu M, Zhang J, Yang M, Zhang H, Xu T, Kan F, Zhang X, Zhang S, Yin Y, Yu F
Journal of cancer research and clinical oncology 2023 Sep;149(11):8429-8442

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HSP70 regulates lipid metabolism of decidual macrophages to maintain normal pregnancy.

Yang D, Dai F, Wang L, Cai S, Zhang Y, Diao L, Cheng Y
Journal of reproductive immunology 2023 Mar;156:103829

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A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates.

Suzuki M, Yamamoto Y, Nishijima-Matsunobu A, Kawasaki Y, Shibata H, Omori Y
FEBS open bio 2023 Mar;13(3):434-446

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Wu L, Zhou L, An J, Shao X, Zhang H, Wang C, Zhao G, Chen S, Cui X, Zhang X, Yang F, Li X, Zhang X
Journal of translational medicine 2023 Jun 15;21(1):388

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Exosomal hsa-miR-151a-3p and hsa-miR-877-5p are potential novel biomarkers for predicting bone metastasis in lung cancer.

Zhao K, Jia C, Wang J, Shi W, Wang X, Song Y, Peng C
Aging 2023 Dec 18;15(24):14864-14888

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Precise pancreatic cancer therapy through targeted degradation of mutant p53 protein by cerium oxide nanoparticles.

Zhang H, Zhang W, Hu B, Qin X, Yi T, Ye Y, Huang X, Song Y, Yang Z, Qian J, Zhang Y
Journal of nanobiotechnology 2023 Apr 1;21(1):117

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Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis.

Han B, Yang M, Liu Q, Wang G, Hou J
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Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Luo L, Gong J, Wang Z, Liu Y, Cao J, Qin J, Zuo R, Zhang H, Wang S, Zhao P, Yang D, Zhang M, Wang Y, Zhang J, Zhou Y, Li C, Ni B, Tian Z, Liu M
Bioactive materials 2022 Sep;15:29-43

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A protein-based cGAS-STING nanoagonist enhances T cell-mediated anti-tumor immune responses.

Wang X, Liu Y, Xue C, Hu Y, Zhao Y, Cai K, Li M, Luo Z
Nature communications 2022 Sep 28;13(1):5685

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Cancer-associated fibroblast-secreted miR-421 promotes pancreatic cancer by regulating the SIRT3/H3K9Ac/HIF-1α axis.

Zhou B, Lei JH, Wang Q, Qu TF, Cha LC, Zhan HX, Liu SL, Hu X, Sun CD, Guo WD, Qiu FB, Cao JY
The Kaohsiung journal of medical sciences 2022 Nov;38(11):1080-1092

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Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.

Yang W, Tan S, Yang L, Chen X, Yang R, Oyang L, Lin J, Xia L, Wu N, Han Y, Tang Y, Su M, Luo X, Yang Y, Huang L, Hu Z, Tao Y, Liu L, Jin Y, Wang H, Liao Q, Zhou Y
Molecular therapy oncolytics 2022 Mar 17;24:612-623

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Proteomics, Phosphoproteomics and Mirna Analysis of Circulating Extracellular Vesicles through Automated and High-Throughput Isolation.

Zhang H, Cai YH, Ding Y, Zhang G, Liu Y, Sun J, Yang Y, Zhan Z, Iliuk A, Gu Z, Gu Y, Tao WA
Cells 2022 Jun 29;11(13)

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BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.

Diao H, Wu K, Lan D, Wang D, Zhao J, Huang B, Shao X, Wang R, Tan H, Tang X, Yan M, Zhang Y
Genes 2022 Jul 26;13(8)

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Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease.

Shao J, Jin Y, Shao C, Fan H, Wang X, Yang G
Cellular & molecular biology letters 2021 Aug 10;26(1):36

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Exosomal miR‑663b exposed to TGF‑β1 promotes cervical cancer metastasis and epithelial‑mesenchymal transition by targeting MGAT3.

You X, Wang Y, Meng J, Han S, Liu L, Sun Y, Zhang J, Sun S, Li X, Sun W, Dong Y, Zhang Y
Oncology reports 2021 Apr;45(4)

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Characterization of mRNA Profiles of Exosomes from Diverse Forms of M2 Macrophages.

Yue Y, Huang S, Wu Z, Wang K, Li H, Hou J, Huang X, Luo L, Liu Q, Wu Z
BioMed research international 2020;2020:1585306

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MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat.

Chen Y, Li J, Ma B, Li N, Wang S, Sun Z, Xue C, Han Q, Wei J, Zhao RC
Aging 2020 Sep 23;12(18):18274-18296

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Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.

Singla S, Iwamoto-Stohl LK, Zhu M, Zernicka-Goetz M
Nature communications 2020 Jun 11;11(1):2958

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Interaction of hnRNP K with MAP 1B-LC1 promotes TGF-β1-mediated epithelial to mesenchymal transition in lung cancer cells.

Li L, Yan S, Zhang H, Zhang M, Huang G, Chen M
BMC cancer 2019 Sep 6;19(1):894

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Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion.

Duan X, Wang W, Liu X, Yan H, Dai R, Lin Q
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2015 Dec;35(6):671-8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The HSP70 antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human HSP70. This antibody recognizes human antigen. The HSP70 antibody has been validated for the following applications: ELISA, WB analysis.

Appendix

66183-1-Ig.pdf

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