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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA308905 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-HSPA1A(HSP70) antibody, Loading control
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-HSPA1A(HSP70) antibody, Loading control
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HSPA1A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Comparative Interactomes of VRK1 and VRK3 with Their Distinct Roles in the Cell Cycle of Liver Cancer.
Lee N, Kim DK, Han SH, Ryu HG, Park SJ, Kim KT, Choi KY
Molecules and cells 2017 Sep 30;40(9):621-631
Molecules and cells 2017 Sep 30;40(9):621-631
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (whole cell lysate). A: 293T 20ug. B: 293T 10ug. C: 293T 5ug. D: 293T 1ug. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: 293T. B: NIH-3T3. C: Mouse brain. D: PC-12. E: Rat brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A(TA308905) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. HSP70 1A antibody (TA308905) dilution: 1:500.
- Validation comment
- IHC
