NB110-96425

antibody from Novus Biologicals

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Immunoelectron microscopy (Data presented on provider website)

Antibody Data

Product number

NB110-96425

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-96425, RRID:AB_1262360

Product name

Mouse Monoclonal HSP70/HSPA1A Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 72 (HSP) and approx 73kDa (HSC).

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Mouse

Isotype

IgG

Vial size

0.2 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Iron deficiency-induced loss of skeletal muscle mitochondrial proteins and respiratory capacity; the role of mitophagy and secretion of mitochondria-containing vesicles.

Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6703-6717

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Identification of Two Kinase Inhibitors with Synergistic Toxicity with Low-Dose Hydrogen Peroxide in Colorectal Cancer Cells in vitro.

Freund E, Liedtke KR, Miebach L, Wende K, Heidecke A, Kaushik NK, Choi EH, Partecke LI, Bekeschus S
Cancers 2020 Jan 2;12(1)

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Hsp70 / Hsc70 Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (N27F3-4) [NB110-96425] - analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.