M00949

antibody from Boster Biological Technology

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00949

Provider

Boster Biological Technology

Product name

Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HSP70/HSPA1A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HSP70/HSPA1A information: Molecular Weight: 70052 MW; Subcellular Localization: Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DGE-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

A multifunctional 'golden cicada' nanoplatform breaks the thermoresistance barrier to launch cascade augmented synergistic effects of photothermal/gene therapy.

Yang W, Wang N, Yang J, Liu C, Ma S, Wang X, Li W, Shen M, Wu Q, Gong C
Journal of nanobiotechnology 2023 Jul 17;21(1):228

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Differentially expressed microRNA profiles in exosomes from vascular smooth muscle cells associated with coronary artery calcification.

Pan W, Liang J, Tang H, Fang X, Wang F, Ding Y, Huang H, Zhang H
The international journal of biochemistry & cell biology 2020 Jan;118:105645

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Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala.

Chen N, Wan XL, Huang CX, Wang WM, Liu H, Wang HL
Immunobiology 2014 Nov;219(11):850-8

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Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells.

Wu YF, Cao MF, Gao YP, Chen F, Wang T, Zumbika EP, Qian KX
World journal of gastroenterology 2004 Oct 15;10(20):2944-8

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody (M00949)HSPA1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA1A Antibody (M00949)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image