Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- Ab108897 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Hsp70 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Hsp70 Antibody, Monoclonal (2#), could be used for WB, IHC, ICC, IF and so on. Application: WB: 0.01-3µg/mL IHC: 5-20µg/mL ICC/IF: 5-20µg/mL Protein Function In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.Tissue specificity: HSPA1B is testis-specific.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-641
- Antibody clone number
- 2#
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Hsp70 Mouse mAb (Ab108897) - Western Blot All lanes: Hsp70 Mouse mAb (Ab108897) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 0.8 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Hsp70 Mouse mAb (Ab108897) - IF/ICC FITC staining on IF Samples: Human Hep G2 cell Primary Ab: 20 μg/mL Hsp70 Mouse mAb (Ab108897) Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Hsp70 Mouse mAb (Ab108897) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Hsp70 Mouse mAb (Ab108897) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.