AM03139PU-N

antibody from Acris Antibodies GmbH

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

AM03139PU-N

Provider

Acris Antibodies GmbH

Proper citation

Product name

anti Heat shock protein 70 / HSP70

Antibody type

Monoclonal

Antigen

Human HSP70 (Ref.1).

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

C92F3A-5

Vial size

50 µg

Concentration

1.0 mg/ml

References

Submitted references

Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis.

Guess AJ, Ayoob R, Chanley M, Manley J, Cajaiba MM, Agrawal S, Pengal R, Pyle AL, Becknell B, Kopp JB, Ronkina N, Gaestel M, Benndorf R, Smoyer WE
PloS one 2013;8(1):e54239

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Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells.

Bauckman KA, Haller E, Flores I, Nanjundan M
Cell death & disease 2013 Apr 18;4:e592

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Effects of corticosteroids in the development of limb muscle weakness in a porcine intensive care unit model.

Aare S, Radell P, Eriksson LI, Akkad H, Chen YW, Hoffman EP, Larsson L
Physiological genomics 2013 Apr 16;45(8):312-20

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Hsp70 in cell lysates from 12 Human cancer cell lines using HSP70 antibody Cat.-No AM03139PU at 1/1000 dilution.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis using HSP70 Antibody Cat.-No AM03139PU-N (Clone C92F3A-5) at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20 µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human colon tissue stained (brown) in inflammatory cells using HSP70 antibody Cat.-No AM03139PU at 1/100,000 dilution.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AM03139PU HSP70 antibody staining of Mouse Melanoma cells