Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA040774 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040774, RRID:AB_10795626
- Product name
- Anti-GIF
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GIF, Gene description: gastric intrinsic factor (vitamin B synthesis), Alternative Gene Names: IF, IFMH, INF, TCN3, Validated applications: IHC, WB, Uniprot ID: P27352, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references p57Kip2 imposes the reserve stem cell state of gastric chief cells
Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia
Wnt/β-catenin promotes gastric fundus specification in mice and humans
Lee J, Kim S, Han S, Min J, Caldwell B, Bamford A, Rocha A, Park J, Lee S, Wu S, Lee H, Fink J, Pilat-Carotta S, Kim J, Josserand M, Szep-Bakonyi R, An Y, Ju Y, Philpott A, Simons B, Stange D, Choi E, Koo B, Kim J
Cell Stem Cell 2022;29(5):826-839.e9
Cell Stem Cell 2022;29(5):826-839.e9
Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia
Puri P, Grimmett G, Faraj R, Gibson L, Gilbreath E, Yoder B
Cellular and Molecular Gastroenterology and Hepatology 2022;14(3):643-668.e1
Cellular and Molecular Gastroenterology and Hepatology 2022;14(3):643-668.e1
Wnt/β-catenin promotes gastric fundus specification in mice and humans
McCracken K, Aihara E, Martin B, Crawford C, Broda T, Treguier J, Zhang X, Shannon J, Montrose M, Wells J
Nature 2017;541(7636):182-187
Nature 2017;541(7636):182-187
No comments: Submit comment
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and GIF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417460).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human stomach and skin tissues using HPA040774 antibody. Corresponding GIF RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
